Quantifying X-ray radiation damage in protein crystals
نویسندگان
چکیده
منابع مشابه
Quantifying X-ray radiation damage in protein crystals at cryogenic temperatures.
The dependence of radiation damage to protein crystals at cryogenic temperatures upon the X-ray absorption cross-section of the crystal has been examined. Lysozyme crystals containing varying heavy-atom concentrations were irradiated and diffraction patterns were recorded as a function of the total number of incident photons. An experimental protocol and a coefficient of sensitivity to absorbed...
متن کاملHow does radiation damage in protein crystals depend on X-ray dose?
Is radiation damage to cryopreserved protein crystals strictly proportional to accumulated dose at the high-flux density of beams from undulators at third-generation synchrotron sources? The answer is "yes," for overall damage to several different kinds of protein crystals at flux densities up to 10(15) ph/sec/mm(2) (APS beamline 19-ID). We find that, at 12 keV (1 A wavelength), about ten absor...
متن کاملNotes from the Bench How does Radiation Damage in Protein Crystals Depend on X-Ray Dose?
diffusion altogether, secondary damage becomes insigIs radiation damage to cryopreserved protein crystals nificant. Crystal decay should then be proportional to strictly proportional to accumulated dose at the highcumulative dose, up to fluxes at which heating rates or flux density of beams from undulators at third-generarelated phenomena are too great to control. Preliminary tion synchrotron s...
متن کاملRadiation damage in protein crystals is reduced with a micron-sized X-ray beam.
Radiation damage is a major limitation in crystallography of biological macromolecules, even for cryocooled samples, and is particularly acute in microdiffraction. For the X-ray energies most commonly used for protein crystallography at synchrotron sources, photoelectrons are the predominant source of radiation damage. If the beam size is small relative to the photoelectron path length, then th...
متن کاملPrimary radiation damage of protein crystals by an intense synchrotron X-ray beam.
X-ray radiation damage of a lysozyme single crystal by an intense monochromatic beam from a third-generation radiation source at the Advanced Photon Source has been studied. The results show that primary radiation damage is linearly dependent on the X-ray dose even when the crystal is at cryogenic temperatures. The existence of an upper limit for the primary radiation damage was observed. Above...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 2007
ISSN: 0108-7673
DOI: 10.1107/s0108767307098078